miR-142和miR-144靶向FoxO1基因调节绵羊前体脂肪细胞分化的研究

doi:10.11843/j.issn.0366-6964.2018.04.003

Abstract

Abstract:

This study aimed to predict and validate the crucial miRNAs that regulate FoxO1 gene expression, and to elucidate the functional mechanism of these miRNAs in ovine adipose differentiation.Four online softwares were used to predict the miRNAs that might target FoxO1. A dual-luciferase reporter assay system was employed to detect luciferase activity. qPCR, Western blotting and Oil red O staining were used to detect the effect of these miRNAs on the expression of FoxO1 both at mRNA and protein levels, and on the regulation of ovine preadipocyte differentiation. The results showed that FoxO1 was targeted by both miR-142 and miR-144.Overexpression of miR-142 or miR-144 significantly inhibited the luciferase activity of the dual-luciferase reporter vector, and reduced the expression of FoxO1 mRNA (P<0.01) and protein (P<0.05). Both miR-142 and miR-144 promoted the differentiation of ovine preadipocytes and accelerated the formation of lipid droplets. In conclusion, miR-142 and miR-144 can down-regulate the expression of FoxO1, and further down-regulate PPARγ expression,thus promote the differentiation of ovine preadipocytes.

CLC Number:

S826.2

ZHAO Yan-yan, QIAO Li-ying, JING Jiong-jie, PAN Yang-yang, REN Duan-yang, WANG Shu-fang, Lü Xuan-zeng, LIU Wen-zhong. Study on the Differentiation Regulation of miR-142 and miR-144 on Ovine Preadipocytes by Targeting FoxO1 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 675-684.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2018.04.003

https://www.xmsyxb.com/EN/Y2018/V49/I4/675

References

[1] SON Y H, KA S, KIM A Y, et al. Regulation of adipocyte differentiation via microRNAs[J]. Endocrinol Metab (Seoul), 2014, 29(2):122-135.
[2] USHMOROV A, WIRTH T. FOXO in B-cell lymphopoiesis and B cell neoplasia[J]. Semin Cancer Biol, 2017, doi:10.1016/j.semcancer.2017.07.008.
[3] WANG Q R, REN J. mTOR-Independent autophagy inducer trehaloserescues against insulin resistance-induced myocardial contractile anomalies:role of p38 MAPK and FoxO1[J]. Pharmacol Res, 2016, 111:357-373.
[4] ROY S K, SRIVASTAVA R K, SHANKAR S. Inhibition of PI3K/AKT and MAPK/ERK pathways causes activation of FoxO transcription factor, leading to cell cycle arrest and apoptosis in pancreatic cancer[J]. J Mol Signal, 2010, 5:10.
[5] PAN C W, JIN X, ZHAO Y, et al. AKT-phosphorylated FOXO1 suppresses ERK activation and chemo-resistance by disrupting IQGAP1-MAPK interaction[J]. EMBO J, 2017, 36(8):995-1010.
[6] YUAN K, AI W B, WAN L Y, et al. The miR-290-295 cluster as multi-faceted players in mouse embryonic stem cells[J]. Cell Biosci, 2017, 7:38.
[7] HILTON C, NEVILLE M J, KARPE F. microRNAs in adipose tissue:their role in adipogenesis and obesity[J]. Int J Obes (Lond), 2013, 37(3):325-332.
[8] LEE E K, LEE M J, ABDELMOHSEN K, et al. miR-130 suppresses adipogenesis by inhibiting peroxisome proliferator-activated receptor γ expression[J]. Mol Cell Biol, 2011, 31(4):626-638.
[9] MOHAMMED B T, SONTAKKE S D, IOANNIDIS J, et al. The adequate corpus luteum:miR-96 promotes luteal cell survival and progesterone production[J]. J Clin Endocrinol Metab, 2017, 102(7):2188-2198.
[10] ZHANG L Q, QUAN H Y, WANG S H, et al. miR-183 promotes growth of non-small cell lung cancer cells through FoxO1 inhibition[J]. Tumor Biol, 2015, 36(10):8121-8126.
[11] WU Z Q, HE B F, HE J C, et al. Upregulation of miR-153 promotes cell proliferation via downregulation of the PTEN tumor suppressor gene in human prostate cancer[J]. Prostate, 2013, 73(6):596-604.
[12] HE W B, FENG L, XIA D L, et al. miR-374a promotes the proliferation of human osteosarcoma by downregulating FOXO1 expression[J]. Int J Clin Exp Med, 2015, 8(3):3482-3489.
[13] XIONG Y, PANG W J, WEI N, et al. Knockdown of both FoxO1 and C/EBPβ promotes adipogenesis in porcine preadipocytes through feedback regulation[J]. Cell Biol Int, 2013, 37(9):905-916.
[14] BABA S, UENO Y, KIKUCHI T, et al. A limonoid kihadanin B from immature Citrus unshiu peels suppresses adipogenesis through repression of the Akt-FOXO1-PPARγ axis in adipocytes[J]. J Agric Food Chem, 2016, 64(51):9607-9615.
[15] LEWIS B P, SHIH I H, JONES-RHOADES M W, et al. Prediction of mammalian microRNA targets[J]. Cell, 2003, 115(7):787-798.
[16] KERTESZ M, IOVINO N, UNNERSTALL U, et al. The role of site accessibility in microRNA target recognition[J]. Nat Genet, 2007, 39(10):1278-1284.
[17] LI J H, LIU S, ZHOU H, et al. StarBase v2.0:decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data[J]. Nucleic Acids Res, 2014, 42(D1):D92-D97.
[18] JOHN B, ENRIGHT A J, ARAVIN A, et al. Human microRNA targets[J]. PLoS Biol, 2004, 2(11):e363.
[19] LIN G L, CHAI J, YUAN S, et al. VennPainter:a tool for the comparison and identification of candidate genes based on Venn diagrams[J]. PLoS One, 2016, 11(4):e0154315.
[20] ZHANG T, KIM D H, XIAO X W, et al. FoxO1 plays an important role in regulating β-cell compensation for insulin resistance in male mice[J]. Endocrinology, 2016, 157(3):1055-1070.
[21] NERURKAR P V, NISHIOKA A, ECK P O, et al. Regulation of glucose metabolism via hepatic forkhead transcription factor 1(FoxO1) by Morinda citrifolia (noni) in high-fat diet-induced obese mice[J]. Br J Nutr, 2012, 108(2):218-228.
[22] KUO Y T, LIN T H, CHEN W L, et al. Alpha-lipoic acid induces adipose triglyceride lipase expression and decreases intracellular lipid accumulation in HepG2 cells[J]. Eur J Pharmacol, 2012, 692(1-3):10-18.
[23] PANG W J, YU T Y, BAI L, et al. Tissue expression of porcine FoxO1 and its negative regulation during primary preadipocyte differentiation[J]. Mol Biol Rep, 2009, 36(1):165-176.
[24] LIU X M, LIU G F, TAN X W, et al. Gene expression profiling of SIRT1, FoxO1, and PPARγ in backfat tissues and subcutaneous adipocytes of Lilu bulls[J]. Meat Sci, 2014, 96(2):704-711.
[25] PU Y, VEIGA-LOPEZ A. PPARγ agonist through the terminal differentiation phase is essential for adipogenic differentiation of fetal ovine preadipocytes[J]. Cell Mol Biol Lett, 2017, 22:6.
[26] 林婄婄, 高中元, 袁亚男, 等. PPARα和PPARγ基因在不同脂尾型绵羊脂肪组织中的发育性表达研究[J]. 畜牧兽医学报, 2012, 43(9):1369-1376.
LIN P P, GAO Z Y, YUAN Y N, et al. Developmental expression of PPARα and PPARγ mRNA in adipose tissues of different fat-tailed sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2012, 43(9):1369-1376. (in Chinese)
[27] DE LA ROSA RODRIGUEZ M A, KERSTEN S. Regulation of lipid droplet-associated proteins by peroxisome proliferator-activated receptors[J]. Biochim Biophys Acta, 2017, 1862(10):1212-1220.
[28] KIM D H, LEE B, KIM M J, et al. Molecular mechanism of betaine on hepatic lipid metabolism:Inhibition of forkhead Box O1(FoxO1) binding to peroxisome proliferator-activated receptor gamma (PPARγ)[J]. J Agric Food Chem, 2016, 64(36):6819-6825.
[29] DONG P Y, MAI Y, ZHANG Z Y, et al. miR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1[J]. Acta Biochim Biophys Sin (Shanghai), 2014, 46(7):565-571.
[30] LI H, XUE M, XU J, et al. miR-301a is involved in adipocyte dysfunction during obesity-related inflammation via suppression of PPARγ[J]. Pharmazie, 2016, 71(2):84-88.
[31] GUO Y T, ZHANG X X, HUANG W L, et al. Identification and characterization of differentially expressed miRNAs in subcutaneous adipose between Wagyu and Holstein cattle[J]. Sci Rep, 2017, 7:44026.
[32] HUANG H Y, LIU R R, ZHAO G P, et al. Integrated analysis of microRNA and mRNA expression profiles in abdominal adipose tissues in chickens[J]. Sci Rep, 2015, 5:16132.

Study on the Differentiation Regulation of miR-142 and miR-144 on Ovine Preadipocytes by Targeting FoxO1 Gene

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	HAN Haoyuan, LI Shikai, YANG Ruiqiao, LI Manman, LI Jun, HA Si, ZHAO Jinyan, WEI Hongfang, QUAN Kai. Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5077-5090.
[3]	LI Dengpan, MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Detection and Analysis of Whole Genome Selection Signal of Yongdeng Qishan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4577-4588.
[4]	ZHAO Xueyang, LI Danni, WANG Yuchen, GUO Lei, WANG Li, HUANG Jie, JIAO Yiqiang, AN Xiaopeng, ZHANG Xiyun, ZHANG Lei, SONG Yuxuan. GWAS Analysis of Lambing Traits in East Friesian and Hu Crossbred Sheep and Verification of Candidate Gene GRID2 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4625-4635.
[5]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[6]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[7]	WANG Wannian, CHEN Sijia, GAO Jinrong, WEN Zhonghao, YUAN Mengjiao, ZHANG Hongzhi, PANG Zhixu, QIAO Liying, LIU Wenzhong. Simulation Study on Genomic Selection of Sex-limited Traits Using Multilayer Perceptron in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2824-2835.
[8]	DONG Yajie, HAO Xiaojing, WU Jinqiang, WANG Rong, ZHANG Pengxiang, WANG Haidong, HE Xiaoyan. Exploration of the Effect of SHH on Wool Bending through Krox20 Regulation of IGFBP5 Expression Based on Sheep Keratinocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2365-2375.
[9]	BI Yazhen, SHANG Mingyu, HU Wenping, ZHANG Li. Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428.
[10]	LI Yinxia, YA Shengjiang·Nasier, SAI Like·Duman, QIAN Yong, CAO Shaoxian, WANG Weilie, MENG Chunhua, ZHANG Jun, ZHANG Jianli. Evaluation of Genetic Diversity and Genetic Structure in Kirgiz Sheep Population Based on SNPs Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 572-583.
[11]	LI Ling, LI Yefang, LIANG Benmeng, SUN Yujiang, MA Yuehui, MA Qing, JIANG Lin, LIU Shuqin. Paternity Identification of Tan Sheep Based on SNP Markers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2912-2919.
[12]	ZHOU Shiwei, WU Tingjie, WANG Qian, CAI Bei, HAN Saizheng, WU Yanhu, WANG Xiaolong, CHEN Yulin. Establishment of the Efficient Method to Detect the FecB Mutation and the Construction of the Prolific Tan Sheep Population [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2920-2929.
[13]	DING Ziqiang, GE Wenbo, DUAN Hongwei, LÜ Jianshu, ZENG Jianlin, WANG Wenjuan, NIU Tian, ZHANG Yong, ZHAO Xingxu, HU Junjie. Dihydrotestosterone Affects Sheep Uterine Function through Regulating Progesterone, Oestrogen and Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2993-3005.
[14]	LI Tingting, LIU Qiuyue, LI Xiangchen, WANG Haitao. Research Progress and Applications of Genes Associated with Economic Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2417-2434.
[15]	XU Songwei, XIANG Hua, ZHANG Huanrong, YANG Lu, WANG Xianjun, SALANG Wenzhu, ZHU Jiangjiang. Effects of Interfering SERBP1 on the Cell Proliferation, Cell Cycle and Apoptosis in Goat Turbinate Bone Primary Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2708-2720.